04337nas a2200169 4500008004100000245018200041210006900223260005400292520365600346653001204002100001804014700002104032700001804053700001804071700002604089856005204115 2015 eng d00aEfficacy of Pseudomonas fluorescens (Pf-CL145A) Spray Dried Powder for Controlling Zebra Mussels Adhering to Test Substrates: U.S. Geological Survey Open-File Report 2015–10510 aEfficacy of Pseudomonas fluorescens PfCL145A Spray Dried Powder  aWashington, D. CbUnited States Geological Survey3 a

A mobile bioassay trailer was used to assess the efficacy of Pseudomonas fluorescens (Pf-CL145A) spray dried powder (SDP) formulation for controlling zebra mussels (Dreissena polymorpha) from two midwestern lakes: Lake Carlos (Alexandria, Minnesota) and Shawano Lake (Shawano, Wisconsin). The effects of SDP exposure concentration and exposure duration on zebra mussel survival were evaluated along with the evaluation of a benthic injection application technique to reduce the amount of SDP required to induce zebra mortality.

Groups of zebra mussels were collected from each lake and allowed to adhere to test substrates for at least 15 days before exposure to SDP. Two independent trials were completed at each lake: (1) a whole water column (WWC) application trial was used to evaluate the effects of SDP exposure concentration and exposure duration on zebra mussel survival; and (2) a benthic injection (BI) application trial in which the SDP was injected into the test tanks to determine the efficacy of a benthic injection application technique to reduce the amount of SDP required to induced zebra mussel mortality. Three exposure durations (6, 9, and 12 hours) were evaluated in the WWC trials and a 12-hour exposure duration was evaluated in the BI trials. All trials contained zebra mussels which were removed at the completion of each exposure duration, consolidated into wire mesh cages, and held in the lake for approximately 30 days before being assessed for survival.

For all trials, treatment was assigned to each test tank according to a randomized block design (n = 3 test tanks per treatment). The treatment groups included (1) an untreated control group, (2) a group that received an application of 50 milligrams of SDP per liter (mg SDP/L), and (3) a group that received an application of 100 mg SDP/L. During the BI trials, SDP was administered to achieve the desired exposure concentration in the bottom 50 percent (175 L) of the test tank. All exposure concentrations are reported as active ingredient.

Approximately 30 days after exposure, zebra mussels were sorted into live and dead, and enumerated. Mean survival of zebra mussels in control treatments exceeded 95 percent. Mean survival of zebra mussels in the Lake Carlos WWC SDP-treated groups ranged from 0.5 to 2.1 percent and when compared at the same exposure duration, no difference was detected in survival between the 50 and 100 milligrams per liter (mg/L) treatment groups. Similarly, mean survival of zebra mussels in the Shawano Lake WWC SDP-treated groups ranged from 2.0 to 12.6 percent and when compared at the same exposure duration, no difference was detected in survival between the 50- and 100-mg/L treatment groups. Mean survival of zebra mussels in the Lake Carlos BI trial SDP-treated groups did not differ (p = 0.93) and was 18.1 and 18.0 percent in the 50- and 100-mg/L treatment groups, respectively. Mean survival of zebra mussels in the Shawano Lake BI trial SDP-treated groups differed (p < 0.01) and was 2.9 and 0.9 percent in the 50- and 100-mg/L treatment groups, respectively. Survival of zebra mussels assigned to the SDP-treated groups in the Lake Carlos WWC trial (12-hour exposure duration) differed from the survival of zebra mussels assigned to the SDP-treated groups in the Lake Carlos BI trial; however, after modification of the BI application technique, no difference (p = 0.22) was detected between the survival of zebra mussel in the Shawano Lake WWC (12-hour exposure duration) and BI trials.

10abiology1 aLuoma, J., A.1 aSeverson, T., J.1 aWeber, K., L.1 aMayer, D., A.1 aNew York State Museum uhttp://pubs.er.usgs.gov/publication/ofr2015105003837nas a2200169 4500008004100000245021500041210006900256260005400325520313300379653001203512100001803524700001803542700002103560700001803581700002603599856004203625 2015 eng d00aEfficacy of Pseudomonas fluorescens Strain CL145A Spray Dried Powder for Controlling Zebra Mussels Adhering to Native Unionid Mussels within Field Enclosures: U.S. Geological Survey Open-File Report 2015–10500 aEfficacy of Pseudomonas fluorescens Strain CL145A Spray Dried Po aWashington, D. CbUnited States Geological Survey3 a

The efficacy of a commercially prepared spray dried powder (SDP) formulation of Pseudomonas fluorescens (strain CL145A) was evaluated for removing zebra mussels (Dreissena polymorpha) adhering to a population of unionid mussels in Lake Darling (Alexandria, Minnesota). Two groups of unionid mussels were used in the study. Unionid mussels were collected near the test area, weighed, photographed, individually tagged, and randomly allocated to one of nine test enclosures in equal proportions and then divided into two groups. The first group of unionid mussels (Group 1, n = 5 per test enclosure) were indiscriminately selected from each test enclosure and used to estimate the number of zebra mussels adhering to unionid mussels prior to exposure. The second group of unionid mussels (Group 2, n = 22 per test enclosure) were used to evaluate the efficacy of SDP for removal of adhering zebra mussels. Both Group 1 and Group 2 mussels were used to evaluate the effects of SDP exposure on unionid mussel survival.

Treatment was assigned to each test enclosure by using a randomized block design. The three treatment groups were tested in triplicate and included an untreated control group and groups that received a single application of 50 or 100 milligrams per liter (mg/L) of SDP based on active ingredient. All treatment concentrations are reported as active ingredient of SDP. Test enclosures were removed at the 8-hour exposure termination. Both Group 1 and Group 2 mussels remained in their assigned exposure location during the postexposure holding period. The number of zebra mussels adhering to Group 2 mussels (live and dead) was assessed 18 to 20 days postexposure in addition to assessing the survival of Group 1 and Group 2 unionid mussels.

SDP, administered as a single treatment, significantly (p < 0.01) reduced the number of adhering zebra mussels when compared to the untreated controls. The number of zebra mussels adhering to unionid mussels (Group 2) was reduced 53 percent in the 50-mg/L treatment group and 68 percent in the 100-mg/L treatment group. The number of adhering zebra mussels did not differ (p = 0.79) between the 50- and 100-mg/L treatment groups after exposure. When standardized to the amount of SDP applied per square meter, each gram (g) of SDP applied in the 50-mg/L treatment reduced the number of adhering zebra mussel 59.8 percent more than the 100-mg/L treatment group.

Group 1 mussel survival did not differ between treatment groups (p > 0.05); however, a difference was detected (p < 0.01) in the survival of Group 2 mussels. The survival of Group 2 mussels did not differ (p > 0.23) between control and treated groups. A difference in Group 2 mussel survival was detected (p = 0.03; odds ratio [OR] = 0.290) between the 50- and 100-mg/L treatment groups (that is, the survival was highest in the 50-mg/L treatment group and lowest in the 100-mg/L treatment group), however, the biological significance of the difference is indeterminate.

10abiology1 aLuoma, J., A.1 aWeber, K., L.1 aSeverson, T., J.1 aMayer, D., A.1 aNew York State Museum uhttp://dx.doi.org/10.3133/ofr2015105103766nas a2200157 4500008004100000245021500041210006900256260002100325520313300346653001203479100001803491700002103509700001803530700001803548856004203566 2015 eng d00aEfficacy of Pseudomonas fluorescens Strain CL145A Spray Dried Powder for Controlling Zebra Mussels Adhering to Native Unionid Mussels Within Field Enclosures: U.S. Geological Survey Open-File Report 2015–10510 aEfficacy of Pseudomonas fluorescens Strain CL145A Spray Dried Po aWashington, D. C3 a

The efficacy of a commercially prepared spray dried powder (SDP) formulation of Pseudomonas fluorescens (strain CL145A) was evaluated for removing zebra mussels (Dreissena polymorpha) adhering to a population of unionid mussels in Lake Darling (Alexandria, Minnesota). Two groups of unionid mussels were used in the study. Unionid mussels were collected near the test area, weighed, photographed, individually tagged, and randomly allocated to one of nine test enclosures in equal proportions and then divided into two groups. The first group of unionid mussels (Group 1, n = 5 per test enclosure) were indiscriminately selected from each test enclosure and used to estimate the number of zebra mussels adhering to unionid mussels prior to exposure. The second group of unionid mussels (Group 2, n = 22 per test enclosure) were used to evaluate the efficacy of SDP for removal of adhering zebra mussels. Both Group 1 and Group 2 mussels were used to evaluate the effects of SDP exposure on unionid mussel survival.

Treatment was assigned to each test enclosure by using a randomized block design. The three treatment groups were tested in triplicate and included an untreated control group and groups that received a single application of 50 or 100 milligrams per liter (mg/L) of SDP based on active ingredient. All treatment concentrations are reported as active ingredient of SDP. Test enclosures were removed at the 8-hour exposure termination. Both Group 1 and Group 2 mussels remained in their assigned exposure location during the postexposure holding period. The number of zebra mussels adhering to Group 2 mussels (live and dead) was assessed 18 to 20 days postexposure in addition to assessing the survival of Group 1 and Group 2 unionid mussels.

SDP, administered as a single treatment, significantly (p < 0.01) reduced the number of adhering zebra mussels when compared to the untreated controls. The number of zebra mussels adhering to unionid mussels (Group 2) was reduced 53 percent in the 50-mg/L treatment group and 68 percent in the 100-mg/L treatment group. The number of adhering zebra mussels did not differ (p = 0.79) between the 50- and 100-mg/L treatment groups after exposure. When standardized to the amount of SDP applied per square meter, each gram (g) of SDP applied in the 50-mg/L treatment reduced the number of adhering zebra mussel 59.8 percent more than the 100-mg/L treatment group.

Group 1 mussel survival did not differ between treatment groups (p > 0.05); however, a difference was detected (p < 0.01) in the survival of Group 2 mussels. The survival of Group 2 mussels did not differ (p > 0.23) between control and treated groups. A difference in Group 2 mussel survival was detected (p = 0.03; odds ratio [OR] = 0.290) between the 50- and 100-mg/L treatment groups (that is, the survival was highest in the 50-mg/L treatment group and lowest in the 100-mg/L treatment group), however, the biological significance of the difference is indeterminate.

10abiology1 aLuoma, J., A.1 aSeverson, T., J.1 aWeber, K., L.1 aMayer, D., A. uhttp://dx.doi.org/10.3133/ofr2015105104259nas a2200157 4500008004100000245021500041210006900256260002100325520362600346653001203972100001803984700001804002700002104020700001804041856004204059 2015 eng d00aEfficacy of Pseudomonas fluorescens Strain CL145A Spray Dried Powder for Controlling Zebra Mussels Adhering to Native Unionid Mussels within Field Enclosures: U.S. Geological Survey Open-File Report 2015–10500 aEfficacy of Pseudomonas fluorescens Strain CL145A Spray Dried Po aWashington, D. C3 a

A mobile bioassay trailer was used to assess the efficacy of Pseudomonas fluorescens (Pf-CL145A) spray dried powder (SDP) formulation for controlling zebra mussels (Dreissena polymorpha) from two midwestern lakes: Lake Carlos (Alexandria, Minnesota) and Shawano Lake (Shawano, Wisconsin). The effects of SDP exposure concentration and exposure duration on zebra mussel survival were evaluated along with the evaluation of a benthic injection application technique to reduce the amount of SDP required to induce zebra mortality.

Groups of zebra mussels were collected from each lake and allowed to adhere to test substrates for at least 15 days before exposure to SDP. Two independent trials were completed at each lake: (1) a whole water column (WWC) application trial was used to evaluate the effects of SDP exposure concentration and exposure duration on zebra mussel survival; and (2) a benthic injection (BI) application trial in which the SDP was injected into the test tanks to determine the efficacy of a benthic injection application technique to reduce the amount of SDP required to induced zebra mussel mortality. Three exposure durations (6, 9, and 12 hours) were evaluated in the WWC trials and a 12-hour exposure duration was evaluated in the BI trials. All trials contained zebra mussels which were removed at the completion of each exposure duration, consolidated into wire mesh cages, and held in the lake for approximately 30 days before being assessed for survival.

For all trials, treatment was assigned to each test tank according to a randomized block design (n = 3 test tanks per treatment). The treatment groups included (1) an untreated control group, (2) a group that received an application of 50 milligrams of SDP per liter (mg SDP/L), and (3) a group that received an application of 100 mg SDP/L. During the BI trials, SDP was administered to achieve the desired exposure concentration in the bottom 50 percent (175 L) of the test tank. All exposure concentrations are reported as active ingredient.

Approximately 30 days after exposure, zebra mussels were sorted into live and dead, and enumerated. Mean survival of zebra mussels in control treatments exceeded 95 percent. Mean survival of zebra mussels in the Lake Carlos WWC SDP-treated groups ranged from 0.5 to 2.1 percent and when compared at the same exposure duration, no difference was detected in survival between the 50 and 100 milligrams per liter (mg/L) treatment groups. Similarly, mean survival of zebra mussels in the Shawano Lake WWC SDP-treated groups ranged from 2.0 to 12.6 percent and when compared at the same exposure duration, no difference was detected in survival between the 50- and 100-mg/L treatment groups. Mean survival of zebra mussels in the Lake Carlos BI trial SDP-treated groups did not differ (p = 0.93) and was 18.1 and 18.0 percent in the 50- and 100-mg/L treatment groups, respectively. Mean survival of zebra mussels in the Shawano Lake BI trial SDP-treated groups differed (p < 0.01) and was 2.9 and 0.9 percent in the 50- and 100-mg/L treatment groups, respectively. Survival of zebra mussels assigned to the SDP-treated groups in the Lake Carlos WWC trial (12-hour exposure duration) differed from the survival of zebra mussels assigned to the SDP-treated groups in the Lake Carlos BI trial; however, after modification of the BI application technique, no difference (p = 0.22) was detected between the survival of zebra mussel in the Shawano Lake WWC (12-hour exposure duration) and BI trials.

10abiology1 aLuoma, J., A.1 aWeber, K., L.1 aSeverson, T., J.1 aMayer, D., A. uhttp://dx.doi.org/10.3133/ofr2015105003160nas a2200181 4500008004100000245021700041210006900258260005400327520242400381653001202805100001802817700001802835700001902853700001702872700001802889700001902907856005202926 2015 eng d00aSafety of Spray-dried Powder Formulated Pseudomonas fluorescens Strain CL145A Exposure to Subadult/Adult Unionid Mussels During Simulated Open-water Treatments: U.S. Geological Survey Open-File Report 2015–10640 aSafety of Spraydried Powder Formulated Pseudomonas fluorescens S aWashington, D. CbUnited States Geological Survey3 a

The exposure effects of a commercially prepared spray dried powder (SDP) formulation ofPseudomonas fluorescens (strain CL145A) on the survival of seven species of unionid mussels endemic to the Great Lakes and Mississippi River basins was evaluated in this study. The study exposures were completed within replicated 350-liter test tanks contained within a mobile bioassay laboratory sited on the shores of the Black River near La Crosse, Wisconsin. The test tanks were supplied with flowing, filtered river water which was interrupted during the exposure period.

Two groups of seven species of mussels were used in equal proportions in the study. The first group was exposed to SDP for 8 hours, and the second group was exposed to SDP for 24 hours. Individually tagged mussels were randomly allocated to test tanks until all test tanks contained 8 to 10 mussels of each species (dependent upon the number available for testing).

The experimental unit for the trial was the individual test tank, and treatment group was assigned using a randomized block design. The treatment groups for each exposure duration consisted of (1) an untreated control group, (2) a group that received an application of 50 milligrams SDP per liter (mg SDP/L), and (3) a group that received an application of 100 mg SDP/L. All mussel species and both exposure duration groups were exposed concurrently (that is, one-half of the mussels were removed after 8 hours of SDP exposure and the remaining mussels were removed after 24 hours of SDP exposure). All treatment concentrations are reported as active ingredient.

After exposure, the mussels were consolidated into wire mesh cages and placed in the Black River for a 27-28 day postexposure period, after which time survival of mussels was assessed. Of the 1,170 mussels tested in the study, 3 were confirmed dead and 5 were not recovered and treated as mortalities in the analysis. The effect and interactions of species, SDP exposure concentration, and SDP exposure duration were analyzed and did not affect mussel survival (p > 0.98). The results from this study indicate that SDP exposure at the maximum approved open-water concentration of 100 mg/L for up to 3 times the maximum approved open-water exposure duration of 8 hours (in other words for 24 hours of exposure) is unlikely to reduce survival of subadult or adult mussels.

10abiology1 aLuoma, J., A.1 aWeber, K., L.1 aWaller, D., L.1 aWise, J., K.1 aMayer, D., A.1 aAloisi, D., B. uhttp://pubs.er.usgs.gov/publication/ofr2015106400747nas a2200169 4500008004100000245021700041210006900258260002100327653001200348100001800360700001800378700001900396700001700415700001800432700001900450856010800469 2015 eng d00aSafety of Spray-dried Powder Formulated Pseudomonas fluorescens Strain CL145A Exposure to Subadult/Adult Unionid Mussels During Simulated Open-water Treatments: U.S. Geological Survey Open-File Report 2015–10640 aSafety of Spraydried Powder Formulated Pseudomonas fluorescens S aWashington, D. C10abiology1 aLuoma, J., A.1 aWeber, K., L.1 aWaller, D., L.1 aWise, J., K.1 aMayer, D., A.1 aAloisi, D., B. uhttps://nysm.nysed.gov/staff-publications/safety-spraydried-powder-formulated-pseudomonas-fluorescens-s